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About this instrument
The controller can be used for single- and multi-use bioreactors from 50 mL (working volume) up to a total volume of 3 L. The current configuration is optimised for cell culture and not suitable for bacterial cultures. For bacterial cultures, see here.
Compatible single-use bioreactors are AppliFlexST (Applikon) or CellReady 3l (Merck).
- 3 channel MFC for precise control of Oxygen, Nitrogen and CO2
- 1 valve control for air overlay
- four peristaltic pumps for adding e.g. base and glucose
- Sensors for dissolved oxygen (DO), pH and temperature
- heating blanket for temperature control
- Lucullus LITE is provided for data logging
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Help and manuals
Workflow overview & check list
- Preparation
- perform two point calibration (pH4 and pH7) of pH sensor
- autoclave pH and DO sensors, liquid addition bottles and tubes
- Polarisation of DO sensor
- fill reaktor vessel with medium (laf bench)
- connect reactor to controller
- air overlay on
- heating blanket on (20%)
- stirrer set on low
- pH sensor can be connected but on hold
- temperature sensor on (fill tube with water)
- leave for least 4h or over night
- perform one-point calibration of DO = 100%
- check pH of medium (be sure that medium tolerates pH)
- Inoculation & start
- connect liquid addition bottles and prime the pumps
- start data logging in lucullus
- move reactor including pH and DO sensors and liquid addition bottles to laf bench
- inoculate with syringe
- reconnect sensors cables and peristaltic pumps
- mount off-gas filter heating
- In Web UI, start all controllers and off gas filter heating
- add a comment in Lucullus ‘inoculation’ to generate time stamp